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KD-Validated Anti-PAFAH1B1 Rabbit Monoclonal Antibody #61494

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Flow cytometric analysis of PAFAH1B1 expression in HepG2 cells using PAFAH1B1 antibody (Cat#61494, 1:2,000). Green, isotype control; red, PAFAH1B1.
Immunocytochemical staining of HepG2 cells with PAFAH1B1 antibody (Cat#61494, 1:1,000). Nuclei were stained blue with DAPI; PAFAH1B1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-PAFAH1B1 antibody (Cat#61494). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PAFAH1B1 antibody (Cat#61494, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). PAFAH1B1, platelet activating factor acetylhydrolase 1b regulatory subunit 1.
Western blotting analysis using anti-PAFAH1B1 antibody (Cat#61494). PAFAH1B1 expression in wild type (WT) and PAFAH1B1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-PAFAH1B1 antibody (Cat#61494, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名：
    PAFAH1B1
  • 货号：
    61494
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-PAFAH1B1 Rabbit Monoclonal Antibody
Aliases PAFAH1B1; Platelet Activating Factor Acetylhydrolase 1b Regulatory Subunit 1; LIS1; PAFAH; MDCR; MDS; Platelet-Activating Factor Acetylhydrolase 1b, Regulatory Subunit 1 (45kDa); Platelet-Activating Factor Acetylhydrolase IB Subunit Beta; NudF; Platelet-Activating Factor Acetylhydrolase, Isoform Ib, Alpha Subunit (45kD); Platelet-Activating Factor Acetylhydrolase, Isoform Ib, Alpha Subunit 45kDa; Platelet-Activating Factor Acetylhydrolase, Isoform Ib, Subunit 1 (45kDa); Miller-Dieker Syndrome Chromosome Region; PAF Acetylhydrolase 45 KDa Subunit; Lissencephaly 1 Protein; Lissencephaly-1 Protein; PAF-AH 45 KDa Subunit; Lissencephaly-1; PAF-AH Alpha; PAFAH Alpha; PAFAHA; LIS-1; LIS2; NUDF
Background

Gene Name: PAFAH1B1

NCBI Gene Entry: 5048

UniProt Entry: P43034
Application Information

Molecular Weight: Predicted, 47 kDa, observed, 47 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB2825

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human LIS1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of PAFAH1B1 expression in HepG2 cells using PAFAH1B1 antibody (Cat#61494, 1:2,000). Green, isotype control; red, PAFAH1B1.
  • Immunocytochemical staining of HepG2 cells with PAFAH1B1 antibody (Cat#61494, 1:1,000). Nuclei were stained blue with DAPI; PAFAH1B1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
  • Western blotting analysis using anti-PAFAH1B1 antibody (Cat#61494). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PAFAH1B1 antibody (Cat#61494, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). PAFAH1B1, platelet activating factor acetylhydrolase 1b regulatory subunit 1.
  • Western blotting analysis using anti-PAFAH1B1 antibody (Cat#61494). PAFAH1B1 expression in wild type (WT) and PAFAH1B1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-PAFAH1B1 antibody (Cat#61494, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
  • 基因名：
    PAFAH1B1
  • 货号：
    61494
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of PAFAH1B1 expression in HepG2 cells using PAFAH1B1 antibody (Cat#61494, 1:2,000). Green, isotype control; red, PAFAH1B1.
Immunocytochemical staining of HepG2 cells with PAFAH1B1 antibody (Cat#61494, 1:1,000). Nuclei were stained blue with DAPI; PAFAH1B1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-PAFAH1B1 antibody (Cat#61494). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PAFAH1B1 antibody (Cat#61494, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). PAFAH1B1, platelet activating factor acetylhydrolase 1b regulatory subunit 1.
Western blotting analysis using anti-PAFAH1B1 antibody (Cat#61494). PAFAH1B1 expression in wild type (WT) and PAFAH1B1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-PAFAH1B1 antibody (Cat#61494, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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