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KD-Validated Anti-Phospho-RSK1(S380) Rabbit Monoclonal Antibody #61513

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Flow cytometric analysis of Phospho-RSK1(S380) expression in C2C12 cells using Phospho-RSK1(S380) antibody (Cat#61513, 1:2,000). Green, isotype control; red, Phospho-RSK1(S380).
Immunocytochemical staining of HeLa cells with Phospho-RSK1(S380) antibody (Cat#61513, 1:1,000). Nuclei were stained blue with DAPI; Phospho-RSK1(S380) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Phospho-RSK1(S380) antibody (Cat#61513). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospho-RSK1(S380) antibody (Cat#61513, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Phospho-RSK1(S380) antibody (Cat#61513). Phospho-RSK1(S380) expression in wild type (WT) and phospho-RSK1(S380) shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Phospho-RSK1(S380) antibody (Cat#61513, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名：
    RPS6KA1
  • 货号：
    61513
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-Phospho-RSK1(S380) Rabbit Monoclonal Antibody
Aliases RPS6KA1; Ribosomal Protein S6 Kinase A1; RSK1; MAPKAPK1; HU-1; MAP Kinase-Activated Protein Kinase 1a; 90 KDa Ribosomal Protein S6 Kinase 1; Ribosomal Protein S6 Kinase Alpha-1; MAPK-Activated Protein Kinase 1a; Ribosomal S6 Kinase 1; MAPKAP Kinase 1a; EC 2.7.11.1; MAPKAPK-1a; MAPKAPK1A; P90-RSK 1; P90RSK1; P90Rsk; P90S6K; RSK-1; DJ590P13.1 (Ribosomal Protein S6 Kinase, 90kD, Polypeptide 1); Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1; Ribosomal Protein S6 Kinase, 90kD, Polypeptide 1; S6K-Alpha 1; S6K-Alpha-1; EC 2.7.11; P90RSK; RSK
Background

Gene Name: RPS6KA1

NCBI Gene Entry: 6195

UniProt Entry: Q15418
Application Information

Molecular Weight: Predicted, 82 kDa, observed, 74 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB2425

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human Phospho-RSK1(S380)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Phospho-RSK1(S380) expression in C2C12 cells using Phospho-RSK1(S380) antibody (Cat#61513, 1:2,000). Green, isotype control; red, Phospho-RSK1(S380).
  • Immunocytochemical staining of HeLa cells with Phospho-RSK1(S380) antibody (Cat#61513, 1:1,000). Nuclei were stained blue with DAPI; Phospho-RSK1(S380) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
  • Western blotting analysis using anti-Phospho-RSK1(S380) antibody (Cat#61513). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospho-RSK1(S380) antibody (Cat#61513, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-Phospho-RSK1(S380) antibody (Cat#61513). Phospho-RSK1(S380) expression in wild type (WT) and phospho-RSK1(S380) shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Phospho-RSK1(S380) antibody (Cat#61513, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
  • 基因名：
    RPS6KA1
  • 货号：
    61513
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of Phospho-RSK1(S380) expression in C2C12 cells using Phospho-RSK1(S380) antibody (Cat#61513, 1:2,000). Green, isotype control; red, Phospho-RSK1(S380).
Immunocytochemical staining of HeLa cells with Phospho-RSK1(S380) antibody (Cat#61513, 1:1,000). Nuclei were stained blue with DAPI; Phospho-RSK1(S380) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Phospho-RSK1(S380) antibody (Cat#61513). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospho-RSK1(S380) antibody (Cat#61513, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Phospho-RSK1(S380) antibody (Cat#61513). Phospho-RSK1(S380) expression in wild type (WT) and phospho-RSK1(S380) shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Phospho-RSK1(S380) antibody (Cat#61513, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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