KD-Validated Anti-SEC24D Rabbit Monoclonal Antibody - 产品中心

KD-Validated Anti-SEC24D Rabbit Monoclonal Antibody #61515

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Flow cytometric analysis of SEC24D expression in C2C12 cells using SEC24D antibody (Cat#61515, 1:2,000). Green, isotype control; red, SEC24D.

Immunocytochemical staining of C2C12 cells with SEC24D antibody (Cat#61515, 1:1,000). Nuclei were stained blue with DAPI; SEC24D was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-SEC24D antibody (Cat#61515). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SEC24D antibody (Cat#61515, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Western blotting analysis using anti-SEC24D antibody (Cat#61515). SEC24D expression in wild type (WT) and SEC24D shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-SEC24D antibody (Cat#61515, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

SEC24D
货号：

61515
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information

Product Name	KD-Validated Anti-SEC24D Rabbit Monoclonal Antibody
Aliases	SEC24D; SEC24 Homolog D, COPII Coat Complex Component; KIAA0755; Protein Transport Protein Sec24D; SEC24-Related Protein D; SEC24 (S. Cerevisiae) Related Gene Family, Member D; SEC24 Family, Member D (S. Cerevisiae); SEC24 Related Gene Family, Member D; SEC24 Family Member D; CLCRP2
Background	Gene Name: SEC24D NCBI Gene Entry: 9871 UniProt Entry: O94855
Application Information	Molecular Weight: Predicted, 113 kDa, observed, 113 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB2510 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human SEC24D
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of SEC24D expression in C2C12 cells using SEC24D antibody (Cat#61515, 1:2,000). Green, isotype control; red, SEC24D.
Immunocytochemical staining of C2C12 cells with SEC24D antibody (Cat#61515, 1:1,000). Nuclei were stained blue with DAPI; SEC24D was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-SEC24D antibody (Cat#61515). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SEC24D antibody (Cat#61515, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-SEC24D antibody (Cat#61515). SEC24D expression in wild type (WT) and SEC24D shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-SEC24D antibody (Cat#61515, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

SEC24D
货号：

61515
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

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