Western blotting analysis using anti-beta actin antibody (Cat#61540). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-beta actin antibody (Cat#61540, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-β-actin antibody (Cat#61540). β-actin expression in wild-type (WT) and ACTB shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-β-actin antibody (Cat#61540, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of β-actin expression in HepG2 cells using anti-β-actin antibody (Cat#61540, 1:2,000). Green, isotype control; red, β-actin.
Immunohistochemistry was performed on paraffin-embedded human lung adenocarcinoma using anti-beta actin antibody (Cat#61540, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
