Flow cytometric analysis of Lamin A/C expression in HeLa cells using anti-Lamin A/C antibody (Cat#61547, 1:2,000). Green, isotype control; red, Lamin A/C.
Western blotting analysis using anti-lamin A/C antibody (Cat#61547). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-lamin A/C antibody (Cat#61547, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-Lamin A/C antibody (Cat#61547). Lamin A/C expression in wild type (WT) and LMNA knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Lamin A/C antibody (Cat#61547, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen
Recombinant protein of human Lamin A/C
Isotype
Mouse IgG1 kappa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:2,500 Flow Cytometry (FCM): 1:2,000
Note
This product is for research use only.
Picture
Flow cytometric analysis of Lamin A/C expression in HeLa cells using anti-Lamin A/C antibody (Cat#61547, 1:2,000). Green, isotype control; red, Lamin A/C.
Western blotting analysis using anti-lamin A/C antibody (Cat#61547). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-lamin A/C antibody (Cat#61547, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-Lamin A/C antibody (Cat#61547). Lamin A/C expression in wild type (WT) and LMNA knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Lamin A/C antibody (Cat#61547, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Lamin A/C expression in HeLa cells using anti-Lamin A/C antibody (Cat#61547, 1:2,000). Green, isotype control; red, Lamin A/C.
Western blotting analysis using anti-lamin A/C antibody (Cat#61547). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-lamin A/C antibody (Cat#61547, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-Lamin A/C antibody (Cat#61547). Lamin A/C expression in wild type (WT) and LMNA knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Lamin A/C antibody (Cat#61547, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
