首页

关于我们

善本标准

产品中心

定制服务

信号通路

促销活动

技术支持

新闻资讯

  • 一抗
  • 二抗
  • 慢病毒
  • 细胞系
  • 裂解物
  • 试剂类
首页>产品中心>一抗

KD-Validated Anti-ATP5B Rabbit Monoclonal Antibody #61585

一键复制产品信息

返回列表

Flow cytometric analysis of ATP5B expression in HepG2 cells using ATP5B antibody (Cat#61585, 1:2,000). Green, isotype control; red, ATP5B.
Immunocytochemical staining of HepG2 cells with anti-ATP5B antibody (Cat#61585, 1:1000) . Nuclei were stained blue with DAPI; ATP5B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Immunohistochemistry was performed on paraffin-embedded mouse kidney using anti-ATP5B antibody (Cat#61585, 1:100). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunohistochemistry was performed on paraffin-embedded mouse brain using anti-ATP5B antibody (Cat#61585, 1:100). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Western blotting analysis using anti-ATP5B antibody (Cat#61585). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ATP5B antibody (Cat#61585, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-ATP5B antibody (Cat#61585). ATP5B expression in wild type (WT) and ATP5B shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-ATP5B antibody (Cat#61585, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of ATP5B knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-ATP5B antibody (Cat#61585, 1:2,000) and analyzed using CytoFLEX.
Immunocytochemical staining of HeLa cells using anti-ATP5B antibody (Cat#61585, 1:1,000), Top panel: wild-type (WT); Bottom panal: ATP5B shRNA knockdown (KD). Nuclei were stained blue with DAPI; ATP5B was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
  • 基因名：
    ATP5F1B
  • 货号：
    61585
  • 应用：
    WB, FCM, IC, IHC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-ATP5B Rabbit Monoclonal Antibody
Aliases ATP5F1B; ATP Synthase F1 Subunit Beta; ATP5B; ATPSB; ATP Synthase, H+ Transporting, Mitochondrial F1 Complex, Beta Polypeptide; ATP Synthase Subunit Beta, Mitochondrial; ATPMB; Mitochondrial ATP Synthetase, Beta Subunit; Mitochondrial ATP Synthase Beta Subunit; Epididymis Secretory Protein Li 271; EC 3.6.3.14; EC 7.1.2.2; HEL-S-271; EC 3.6.3; HUMOP2
Background

Gene Name: ATP5F1B

NCBI Gene Entry: 506

UniProt Entry: P06576
Application Information

Molecular Weight: Predicted, 57 kDa, observed, 50 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB3255

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC), immunohistochemistry (IHC)
Immunogen A synthesized peptide derived from human ATPB
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (IC): 1:100-1:1,000
Flow Cytometry (FCM): 1:2,000
Immunohistochemistry (IHC): 1:50-1:100
Note This product is for research use only.
Picture
  • Flow cytometric analysis of ATP5B expression in HepG2 cells using ATP5B antibody (Cat#61585, 1:2,000). Green, isotype control; red, ATP5B.
  • Immunocytochemical staining of HepG2 cells with anti-ATP5B antibody (Cat#61585, 1:1000) . Nuclei were stained blue with DAPI; ATP5B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Immunohistochemistry was performed on paraffin-embedded mouse kidney using anti-ATP5B antibody (Cat#61585, 1:100). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Immunohistochemistry was performed on paraffin-embedded mouse brain using anti-ATP5B antibody (Cat#61585, 1:100). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Western blotting analysis using anti-ATP5B antibody (Cat#61585). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ATP5B antibody (Cat#61585, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-ATP5B antibody (Cat#61585). ATP5B expression in wild type (WT) and ATP5B shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-ATP5B antibody (Cat#61585, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Validation of ATP5B knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-ATP5B antibody (Cat#61585, 1:2,000) and analyzed using CytoFLEX.
  • Immunocytochemical staining of HeLa cells using anti-ATP5B antibody (Cat#61585, 1:1,000), Top panel: wild-type (WT); Bottom panal: ATP5B shRNA knockdown (KD). Nuclei were stained blue with DAPI; ATP5B was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
Citation(0)
  • 基因名：
    ATP5F1B
  • 货号：
    61585
  • 应用：
    WB, FCM, IC, IHC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of ATP5B expression in HepG2 cells using ATP5B antibody (Cat#61585, 1:2,000). Green, isotype control; red, ATP5B.
Immunocytochemical staining of HepG2 cells with anti-ATP5B antibody (Cat#61585, 1:1000) . Nuclei were stained blue with DAPI; ATP5B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Immunohistochemistry was performed on paraffin-embedded mouse kidney using anti-ATP5B antibody (Cat#61585, 1:100). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunohistochemistry was performed on paraffin-embedded mouse brain using anti-ATP5B antibody (Cat#61585, 1:100). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Western blotting analysis using anti-ATP5B antibody (Cat#61585). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ATP5B antibody (Cat#61585, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-ATP5B antibody (Cat#61585). ATP5B expression in wild type (WT) and ATP5B shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-ATP5B antibody (Cat#61585, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of ATP5B knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-ATP5B antibody (Cat#61585, 1:2,000) and analyzed using CytoFLEX.
Immunocytochemical staining of HeLa cells using anti-ATP5B antibody (Cat#61585, 1:1,000), Top panel: wild-type (WT); Bottom panal: ATP5B shRNA knockdown (KD). Nuclei were stained blue with DAPI; ATP5B was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
  • 联系电话

    0551-63635518

    0551-65578998

  • 联系邮箱

    support@genuinbiotech.cn

    sales@genuinbiotech.cn

微信公众号

Copyright © 2025 合肥善本生物科技有限公司

All Rights Reserved. 皖ICP备2023000814号