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KD-Validated Anti-Phospho-PRAS40 (T246) Rabbit Monoclonal Antibody #61633

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Flow cytometric analysis of Phospho-PRAS40 (T246) expression in HeLa cells using Phospho-PRAS40 (T246) antibody (Cat#61633, 1:2,000). Green, isotype control; red, Phospho-PRAS40 (T246).
Immunocytochemical staining of HeLa cells with Phospho-PRAS40 (T246) antibody (Cat#61633, 1:1,000). Nuclei were stained blue with DAPI; Phospho-PRAS40 (T246) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm.
Western blotting analysis using anti-Phospho-PRAS40 (T246) antibody (Cat#61633). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospho-PRAS40 (T246) antibody (Cat#61633, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Phospho-PRAS40 (T246) antibody (Cat#61633). Phospho-PRAS40 (T246) expression in wild type (WT) and Phospho-PRAS40 (T246) shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Phospho-PRAS40 (T246) antibody (Cat#61633, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of PRAS40 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Phospho-PRAS40 (T246) antibody (Cat#61633, 1:2,000) and analyzed using BD flow cytometer.
  • 基因名：
    AKT1S1
  • 货号：
    61633
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-Phospho-PRAS40 (T246) Rabbit Monoclonal Antibody
Aliases AKT1S1; AKT1 Substrate 1; PRAS40; 40 KDa Proline-Rich AKT Substrate; Proline-Rich AKT1 Substrate 1; MGC2865; Lobe; Proline-Rich Akt Substrate, 40 KDa; AKT1 Substrate 1 (Proline-Rich); AKT1 Substrate 1 (Proline Rich); LOBE
Background

Gene Name: AKT1S1

NCBI Gene Entry: 84335

UniProt Entry: Q96B36
Application Information

Molecular Weight: Predicted, 27 kDa, observed, 40 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB3855

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human Phospho-PRAS40 (T246)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Phospho-PRAS40 (T246) expression in HeLa cells using Phospho-PRAS40 (T246) antibody (Cat#61633, 1:2,000). Green, isotype control; red, Phospho-PRAS40 (T246).
  • Immunocytochemical staining of HeLa cells with Phospho-PRAS40 (T246) antibody (Cat#61633, 1:1,000). Nuclei were stained blue with DAPI; Phospho-PRAS40 (T246) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm.
  • Western blotting analysis using anti-Phospho-PRAS40 (T246) antibody (Cat#61633). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospho-PRAS40 (T246) antibody (Cat#61633, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-Phospho-PRAS40 (T246) antibody (Cat#61633). Phospho-PRAS40 (T246) expression in wild type (WT) and Phospho-PRAS40 (T246) shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Phospho-PRAS40 (T246) antibody (Cat#61633, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Validation of PRAS40 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Phospho-PRAS40 (T246) antibody (Cat#61633, 1:2,000) and analyzed using BD flow cytometer.
Citation(0)
  • 基因名：
    AKT1S1
  • 货号：
    61633
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of Phospho-PRAS40 (T246) expression in HeLa cells using Phospho-PRAS40 (T246) antibody (Cat#61633, 1:2,000). Green, isotype control; red, Phospho-PRAS40 (T246).
Immunocytochemical staining of HeLa cells with Phospho-PRAS40 (T246) antibody (Cat#61633, 1:1,000). Nuclei were stained blue with DAPI; Phospho-PRAS40 (T246) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm.
Western blotting analysis using anti-Phospho-PRAS40 (T246) antibody (Cat#61633). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospho-PRAS40 (T246) antibody (Cat#61633, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Phospho-PRAS40 (T246) antibody (Cat#61633). Phospho-PRAS40 (T246) expression in wild type (WT) and Phospho-PRAS40 (T246) shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Phospho-PRAS40 (T246) antibody (Cat#61633, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of PRAS40 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Phospho-PRAS40 (T246) antibody (Cat#61633, 1:2,000) and analyzed using BD flow cytometer.
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