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KD-Validated Anti-Cyclin Dependent Kinase Inhibitor 1A Rabbit Monoclonal Antibody #61661

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Flow cytometric analysis of p21 expression in HT-1080 cells using anti-p21 antibody (Cat#61661, 1:2,000). Green, isotype control; red, p21.
Immunocytochemical staining of HT-1080 cells with anti-p21 antibody (Cat#61661, 1:1000) . Nuclei were stained blue with DAPI; p21 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-p21 antibody (Cat#61661). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-p21 antibody (Cat#61661, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-p21 antibody (Cat#61661). p21 expression in wild type (WT) and CDKN1A knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-p21 antibody (Cat#61661, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名：
    CDKN1A
  • 货号：
    61661
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-Cyclin Dependent Kinase Inhibitor 1A Rabbit Monoclonal Antibody
Aliases CDKN1A; Cyclin Dependent Kinase Inhibitor 1A; CAP20; CIP1; WAF1; SDI1; P21; P21CIP1; CDKN1; Cyclin-Dependent Kinase Inhibitor 1A (P21, Cip1); Cyclin-Dependent Kinase Inhibitor 1;
Cdk-Interacting Protein 1; P21Cip1/Waf1; MDA-6; Melanoma Differentiation Associated Protein 6; Melanoma Differentiation-Associated Protein 6; Wild-Type P53-Activated Fragment 1; CDK-Interaction Protein 1; CDK-Interacting Protein 1; DNA Synthesis Inhibitor; MDA6; PIC1
Background

Gene Name: CDKN1A

NCBI Gene Entry: 1026

UniProt Entry: P38936
Application Information

Molecular Weight: Predicted, 18 kDa; observed, 18 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB3200

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human p21
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of p21 expression in HT-1080 cells using anti-p21 antibody (Cat#61661, 1:2,000). Green, isotype control; red, p21.
  • Immunocytochemical staining of HT-1080 cells with anti-p21 antibody (Cat#61661, 1:1000) . Nuclei were stained blue with DAPI; p21 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-p21 antibody (Cat#61661). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-p21 antibody (Cat#61661, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-p21 antibody (Cat#61661). p21 expression in wild type (WT) and CDKN1A knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-p21 antibody (Cat#61661, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
  • 基因名：
    CDKN1A
  • 货号：
    61661
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of p21 expression in HT-1080 cells using anti-p21 antibody (Cat#61661, 1:2,000). Green, isotype control; red, p21.
Immunocytochemical staining of HT-1080 cells with anti-p21 antibody (Cat#61661, 1:1000) . Nuclei were stained blue with DAPI; p21 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-p21 antibody (Cat#61661). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-p21 antibody (Cat#61661, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-p21 antibody (Cat#61661). p21 expression in wild type (WT) and CDKN1A knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-p21 antibody (Cat#61661, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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