Flow cytometric analysis of cleaved Caspase-9 expression in HepG2 cells using cleaved Caspase-9 antibody (Cat#61679, 1:2,000). Green, isotype control; red, cleaved Caspase-9.
Immunocytochemical staining of HepG2 cells with cleaved Caspase-9 antibody (Cat#61679, 1:1,000). Nuclei were stained blue with DAPI; cleaved Caspase-9 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-cleaved Caspase-9 antibody (Cat#61679). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-cleaved Caspase-9 antibody (Cat#61679, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-cleaved Caspase-9 antibody (Cat#61679). Cleaved Caspase-9 expression in wild type (WT) and cleaved Caspase-9 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-cleaved Caspase-9 antibody (Cat#61679, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of cleaved Caspase-9 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-cleaved Caspase-9 antibody (Cat#61679, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of HeLa cells using anti-Cleaved Caspase-9 antibody (Cat#61679, 1:1,000), Top panel: wild-type (WT); Bottom panal: Cleaved Caspase-9 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Cleaved Caspase-9 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
