Flow cytometric analysis of Syntaxin 4 expression in HepG2 cells using anti-Syntaxin 4 antibody (Cat#61691, 1:2,000). Green, isotype control; red, Syntaxin 4.
Immunocytochemical staining of Syntaxin 4 cells with Syntaxin 4 antibody (Cat#61691, 1:1,000). Nuclei were stained blue with DAPI; Syntaxin 4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-syntaxin 4 antibody (Cat#61691). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-syntaxin 4 antibody (Cat#61691, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-syntaxin 4 antibody (Cat#61691). Syntaxin 4 expression in wild-type (WT) and syntaxin 4 (STX4) knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-syntaxin 4 antibody (Cat#61691, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
