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KD-Validated Anti-SMARCC1 Rabbit Monoclonal Antibody #61706

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Flow cytometric analysis of SMARCC1 expression in HepG2 cells using SMARCC1 antibody (Cat#61706, 1:2,000). Green, isotype control; red, SMARCC1.
Immunocytochemical staining of HepG2 cells with SMARCC1 antibody (Cat#61706, 1:1,000). Nuclei were stained blue with DAPI; SMARCC1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-SMARCC1 antibody (Cat#61706). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SMARCC1 antibody (Cat#61706, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-SMARCC1 antibody (Cat#61706). SMARCC1 expression in wild type (WT) and SMARCC1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-SMARCC1 antibody (Cat#61706, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名：
    SMARCC1
  • 货号：
    61706
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-SMARCC1 Rabbit Monoclonal Antibody
Aliases SMARCC1; SWI/SNF Related, Matrix Associated, Actin Dependent Regulator Of Chromatin Subfamily C Member 1; BAF155; CRACC1; SRG3; SWI/SNF-Related Matrix-Associated Actin-Dependent Regulator Of Chromatin Subfamily C Member 1; SWI/SNF Complex Subunit SMARCC1; SWI/SNF Complex 155 KDa Subunit; BRG1-Associated Factor 155; Rsc8; SWI/SNF Related, Matrix Associated, Actin Dependent Regulator Of; Chromatin, Subfamily C, Member 1; Mammalian Chromatin Remodeling Complex BRG1-Associated Factor 155; Chromatin Remodeling Complex BAF155 Subunit; HYC5; SWI3; RSC8
Background

Gene Name: SMARCC1

NCBI Gene Entry: 6599

UniProt Entry: Q92922
Application Information

Molecular Weight: Predicted, 123 kDa, observed, 155 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB4165

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human SMARCC1/BAF155
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of SMARCC1 expression in HepG2 cells using SMARCC1 antibody (Cat#61706, 1:2,000). Green, isotype control; red, SMARCC1.
  • Immunocytochemical staining of HepG2 cells with SMARCC1 antibody (Cat#61706, 1:1,000). Nuclei were stained blue with DAPI; SMARCC1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
  • Western blotting analysis using anti-SMARCC1 antibody (Cat#61706). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SMARCC1 antibody (Cat#61706, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-SMARCC1 antibody (Cat#61706). SMARCC1 expression in wild type (WT) and SMARCC1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-SMARCC1 antibody (Cat#61706, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
  • 基因名：
    SMARCC1
  • 货号：
    61706
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of SMARCC1 expression in HepG2 cells using SMARCC1 antibody (Cat#61706, 1:2,000). Green, isotype control; red, SMARCC1.
Immunocytochemical staining of HepG2 cells with SMARCC1 antibody (Cat#61706, 1:1,000). Nuclei were stained blue with DAPI; SMARCC1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-SMARCC1 antibody (Cat#61706). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SMARCC1 antibody (Cat#61706, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-SMARCC1 antibody (Cat#61706). SMARCC1 expression in wild type (WT) and SMARCC1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-SMARCC1 antibody (Cat#61706, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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