Flow cytometric analysis of Phospho-EGFR (Tyr1068) expression in HepG2 cells using anti-Phospho-EGFR (Tyr1068) antibody (Cat#61720, 1:2,000). Green, isotype control; red, Phospho-EGFR (Tyr1068).
Western blotting analysis using anti-phospho-EGFR (Tyr1068) antibody (Cat#61720). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-EGFR (Tyr1068) antibody (Cat#61720, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-phospho-EGFR (Tyr1068) antibody (Cat#61720). Phospho-EGFR (Tyr1068) expression in wild-type (WT) and EGFR shRNA knockdown (KD) HepG2 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-phospho-EGFR (Tyr1068) antibody (Cat#61720, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).