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KD-Validated Anti-L1 Cell Adhesion Molecule Rabbit Monoclonal Antibody #61742

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Flow cytometric analysis of L1 cell adhesion molecule expression in HepG2 cells using anti-L1 cell adhesion molecule antibody (Cat#61742, 1:2,000). Green, isotype control; red, L1 cell adhesion molecule.
Western blotting analysis using anti-L1 cell adhesion molecule antibody (Cat#61742). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-L1 cell adhesion molecule antibody (Cat#61742, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-L1 cell adhesion molecule antibody (Cat#61742). L1 cell adhesion molecule expression in wild type (WT) and L1 cell adhesion molecule (L1CAM) knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-L1 cell adhesion molecule antibody (Cat#61742, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名:
    L1CAM
  • 货号:
    61742
  • 应用:
    WB, FCM
  • 物种反应性:
    H
规格:
20 μL,50 μL,100 μL
价格:
¥ 650.00,¥ 1,350.00,¥ 2,500.00
Information
Product Name KD-Validated Anti-L1 Cell Adhesion Molecule Rabbit Monoclonal Antibody
Aliases L1CAM; L1 Cell Adhesion Molecule; NCAM-L1; CAML1; Neural Cell Adhesion Molecule L1; CD171; MIC5; Antigen Identified By Monoclonal Antibody R1; N-CAM-L1; HSAS1; HSAS; MASA; SPG1; S10; CD171 Antigen; N-CAML1; HYCX
Background

Gene Name: L1CAM

NCBI Gene Entry: 3897

UniProt Entry: P32004

Application Information

Molecular Weight: Predicted, 140 kDa; observed, 80,220 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB3510

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen Recombinant protein of human L1CAM
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of L1 cell adhesion molecule expression in HepG2 cells using anti-L1 cell adhesion molecule antibody (Cat#61742, 1:2,000). Green, isotype control; red, L1 cell adhesion molecule.
  • Western blotting analysis using anti-L1 cell adhesion molecule antibody (Cat#61742). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-L1 cell adhesion molecule antibody (Cat#61742, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using anti-L1 cell adhesion molecule antibody (Cat#61742). L1 cell adhesion molecule expression in wild type (WT) and L1 cell adhesion molecule (L1CAM) knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-L1 cell adhesion molecule antibody (Cat#61742, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
Flow cytometric analysis of L1 cell adhesion molecule expression in HepG2 cells using anti-L1 cell adhesion molecule antibody (Cat#61742, 1:2,000). Green, isotype control; red, L1 cell adhesion molecule.
Western blotting analysis using anti-L1 cell adhesion molecule antibody (Cat#61742). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-L1 cell adhesion molecule antibody (Cat#61742, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-L1 cell adhesion molecule antibody (Cat#61742). L1 cell adhesion molecule expression in wild type (WT) and L1 cell adhesion molecule (L1CAM) knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-L1 cell adhesion molecule antibody (Cat#61742, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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