Flow cytometric analysis of CBR1 expression in HeLa cells using CBR1 antibody (Cat#61765, 1:2,000). Green, isotype control; red, CBR1.
Immunocytochemical staining of HeLa cells with anti-CBR1 antibody (Cat#61765, 1:1,000). Nuclei were stained blue with DAPI; CBR1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-carbonyl reductase 1 antibody (Cat#61765). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-carbonyl reductase 1 antibody (Cat#61765, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-carbonyl reductase 1 antibody (Cat#61765). Carbonyl reductase 1 expression in wild-type (WT) and carbonyl reductase 1 (CBR1) knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-carbonyl reductase 1 antibody (Cat#61765, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
