Flow cytometric analysis of Caspase 9 expression in HepG2 cells using Caspase 9 antibody (Cat#61769, 1:2,000). Green, isotype control; red, Caspase 9.
Immunocytochemical staining of HepG2 cells with caspase 9 antibody (Cat#61769, 1:1000). Nuclei were stained blue with DAPI; Caspase 9 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: medium. Scale bar: 20 μm.
Western blotting analysis using anti-Caspase 9 antibody (Cat#61769). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Caspase 9 antibody (Cat#61769, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Caspase 9 antibody (Cat#61769). Caspase 9 expression in wild type (WT) and Caspase 9 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Caspase 9 antibody (Cat#61769, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of Caspase 9 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Caspase 9 antibody (Cat#61769, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of HeLa cells using anti-Caspase 9 antibody (Cat#61769, 1:1,000), Top panel: wild-type (WT); Bottom panal: Caspase 9 shRNA knockdown (KD). Nuclei were stained blue with DAPI;Caspase 9 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
