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KD-Validated Anti-ERK1/2 Rabbit Monoclonal Antibody #61797

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Flow cytometric analysis of ERK1/2 expression in C2C12 cells using ERK1/2 antibody (Cat#61797, 1:2,000). Green, isotype control; red, ERK1/2.
Immunocytochemical staining of C2C12 cells with ERK1/2 antibody (Cat#61797, 1:1,000). Nuclei were stained blue with DAPI; ERK1/2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-ERK1/2 antibody (Cat#61797). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ERK1/2 antibody (Cat#61797, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-ERK1/2 antibody (Cat#61797). ERK1/2 expression in wild type (WT) and ERK1/2 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-ERK1/2 antibody (Cat#61797, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名：
    MAPK1/3
  • 货号：
    61797
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-ERK1/2 Rabbit Monoclonal Antibody
Aliases MAPK3; Mitogen-Activated Protein Kinase 3; ERK1; PRKM3; Extracellular Signal-Regulated Kinase 1; Microtubule-Associated Protein 2 Kinase; Insulin-Stimulated MAP2 Kinase; EC 2.7.11.24; P44-ERK1; P44-MAPK; P44ERK1; P44MAPK; ERK-1; ERT2; Extracellular Signal-Related Kinase 1; MAP Kinase Isoform P44; MAP Kinase; EC 2.7.11; HS44KDAP; HUMKER1A; P44mapk; P44erk1; MAPK 1; MAPK 3; MAPK1; Mitogen-Activated Protein Kinase 1; ERK2; Extracellular Signal-Regulated Kinase 2; MAPK2; PRKM1; PRKM2; ERK; Mitogen-Activated Protein Kinase 2; MAP Kinase 1; MAP Kinase 2; EC 2.7.11.24; P42-MAPK; P41mapk; MAPK; ERK-2; ERT1; Protein Tyrosine Kinase; ERK2; MAP Kinase Isoform P42; EC 2.7.11; P42MAPK; P41MAPK; MAPK 1; NS13; P38; P40; P41
Background

Gene Name: MAPK1/3

NCBI Gene Entry: 5595/5594

UniProt Entry: P27361/ P28482
Application Information

Molecular Weight: Predicted, 43,41 kDa, observed, 42,44 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB4510

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human ERK1/2
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of ERK1/2 expression in C2C12 cells using ERK1/2 antibody (Cat#61797, 1:2,000). Green, isotype control; red, ERK1/2.
  • Immunocytochemical staining of C2C12 cells with ERK1/2 antibody (Cat#61797, 1:1,000). Nuclei were stained blue with DAPI; ERK1/2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
  • Western blotting analysis using anti-ERK1/2 antibody (Cat#61797). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ERK1/2 antibody (Cat#61797, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-ERK1/2 antibody (Cat#61797). ERK1/2 expression in wild type (WT) and ERK1/2 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-ERK1/2 antibody (Cat#61797, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
  • 基因名：
    MAPK1/3
  • 货号：
    61797
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of ERK1/2 expression in C2C12 cells using ERK1/2 antibody (Cat#61797, 1:2,000). Green, isotype control; red, ERK1/2.
Immunocytochemical staining of C2C12 cells with ERK1/2 antibody (Cat#61797, 1:1,000). Nuclei were stained blue with DAPI; ERK1/2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-ERK1/2 antibody (Cat#61797). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ERK1/2 antibody (Cat#61797, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-ERK1/2 antibody (Cat#61797). ERK1/2 expression in wild type (WT) and ERK1/2 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-ERK1/2 antibody (Cat#61797, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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