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KD-Validated Anti-ATP6V1A Rabbit Monoclonal Antibody #61817

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Flow cytometric analysis of ATP6V1A expression in HepG2 cells using anti-ATP6V1A antibody (Cat#61817, 1:2,000). Green, isotype control; red, ATP6V1A.
Immunocytochemical staining of HepG2 cells with anti-ATP6V1A antibody (Cat#61817, 1:1,000). Nuclei were stained blue with DAPI; ATP6V1A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-ATP6V1A antibody (Cat#61817). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ATP6V1A antibody (Cat#61817, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-ATP6V1A antibody (Cat#61817). ATP6V1A expression in wild-type (WT) and ATP6V1A knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-ATP6V1A antibody (Cat#61817, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名：
    ATP6V1A
  • 货号：
    61817
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-ATP6V1A Rabbit Monoclonal Antibody
Aliases ATP6V1A; ATPase H+ Transporting V1 Subunit A; V-ATPase Subunit A; ATP6V1A1; ATP6A1; Vma1; VA68; VPP2; ATPase, H+ Transporting, Lysosomal 70kDa, V1 Subunit A; V-Type Proton ATPase (V-ATPase) Catalytic Subunit A; V-Type Proton ATPase Catalytic Subunit A; Vacuolar Proton Pump Subunit Alpha; ATPase, H+ Transporting, Lysosomal (Vacuolar Proton Pump), Alpha Polypeptide, 70kD, Isoform 1; H+-Transporting ATPase Chain A, Vacuolar (VA68 Type); ATPase, H+ Transporting, Lysosomal, Subunit A1; H(+)-Transporting Two-Sector ATPase, Subunit A; Vacuolar Proton Pump Alpha Subunit 1; Vacuolar ATPase Isoform VA68; V-ATPase 69 KDa Subunit 1; Vacuolar-Type H(+)-ATPase; V-ATPase 69 KDa Subunit; V-ATPase A Subunit 1; EC 3.6.3.14; EC 7.1.2.2; EC 3.6.3; ARCL2D; IECEE3; DEE93; HO68
Background

Gene Name: ATP6V1A

NCBI Gene Entry: 523

UniProt Entry: P38606
Application Information

Molecular Weight: Predicted, 68 kDa; observed, 68 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB3865

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen Recombinant protein of human ATP6V1A
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of ATP6V1A expression in HepG2 cells using anti-ATP6V1A antibody (Cat#61817, 1:2,000). Green, isotype control; red, ATP6V1A.
  • Immunocytochemical staining of HepG2 cells with anti-ATP6V1A antibody (Cat#61817, 1:1,000). Nuclei were stained blue with DAPI; ATP6V1A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-ATP6V1A antibody (Cat#61817). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ATP6V1A antibody (Cat#61817, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using anti-ATP6V1A antibody (Cat#61817). ATP6V1A expression in wild-type (WT) and ATP6V1A knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-ATP6V1A antibody (Cat#61817, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
  • 基因名：
    ATP6V1A
  • 货号：
    61817
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of ATP6V1A expression in HepG2 cells using anti-ATP6V1A antibody (Cat#61817, 1:2,000). Green, isotype control; red, ATP6V1A.
Immunocytochemical staining of HepG2 cells with anti-ATP6V1A antibody (Cat#61817, 1:1,000). Nuclei were stained blue with DAPI; ATP6V1A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-ATP6V1A antibody (Cat#61817). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ATP6V1A antibody (Cat#61817, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-ATP6V1A antibody (Cat#61817). ATP6V1A expression in wild-type (WT) and ATP6V1A knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-ATP6V1A antibody (Cat#61817, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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