Immunohistochemistry was performed on paraffin-embedded human esophageal carcinoma using anti-caspase 8 antibody (Cat#61830, 1:100). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Flow cytometric analysis of Caspase 8 expression in HeLa cells using anti-Caspase 8 antibody (Cat#61830, 1:2,000). Green, isotype control; red, Caspase 8.
Western blotting analysis using anti-caspase 8 antibody (Cat#61830). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-caspase 8 antibody (Cat#61830, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-caspase 8 antibody (Cat#61830). Caspase 8 expression in wild-type (WT) and caspase 8 (CASP8) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-caspase 8 antibody (Cat#61830, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
