Flow cytometric analysis of BAG5 expression in C2C12 cells using anti-BAG5 antibody (Cat#61841, 1:1,000). Green, isotype control; red, BAG5.
Immunocytochemical staining of C2C12 cells with anti-BAG5 antibody (Cat#61841, 1:1,000). Nuclei were stained blue with DAPI; BAG5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-BAG5 antibody (Cat#61841). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-BAG5 antibody (Cat#61841, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-BAG5 antibody (Cat#61841). BAG5 expression in wild type (WT) and BAG5 shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-BAG5 antibody (Cat#61841, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
