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KD-Validated Anti-RUNX1/RUNX2/RUNX3 Rabbit Monoclonal Antibody #61851

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Flow cytometric analysis of RUNX1/RUNX2/RUNX3 expression in HT-1080 cells using RUNX1/RUNX2/RUNX3 antibody (Cat#61851, 1:2,000). Green, isotype control; red, RUNX1/RUNX2/RUNX3.
Immunocytochemical staining of HT-1080 cells with anti-RUNX1/RUNX2/RUNX3 antibody (Cat #61851, 1:1,000) . Nuclei were stained blue with DAPI; RUNX1/RUNX2/RUNX3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-RUNX1/RUNX2/RUNX3 antibody (Cat#61851). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RUNX1/RUNX2/RUNX3 antibody (Cat#61851, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-RUNX1/RUNX2/RUNX3 antibody (Cat#61851). RUNX1/RUNX2/RUNX3 expression in wild type (WT) and RUNX1/RUNX2/RUNX3 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-RUNX1/RUNX2/RUNX3 antibody (Cat#61851, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名：
    RUNX1/RUNX2/RUNX3
  • 货号：
    61851
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-RUNX1/RUNX2/RUNX3 Rabbit Monoclonal Antibody
Aliases RUNX1; RUNX Family Transcription Factor 1; Runt-Related Transcription Factor 1; AMLCR1; CBFA2; AML1; Polyomavirus Enhancer-Binding Protein 2 Alpha B Subunit; SL3/AKV Core-Binding Factor Alpha B Subunit; SL3-3 Enhancer Factor 1 Alpha B Subunit; Runt Related Transcription Factor 1; Acute Myeloid Leukemia 1 Protein; Oncogene AML-1; PEBP2-Alpha B; PEA2-Alpha B; PEBP2A2; Core-Binding Factor, Runt Domain, Alpha Subunit; Core-Binding Factor Subunit Alpha-2; AML1-EVI-1 Fusion Protein; Acute Myeloid Leukemia 1; AML1-ETO Fusion Protein; AML1-ETO Fusion; Aml1 Oncogene; Mutant RUNX1; CBF-Alpha-2; AML1-EVI-1; PEBP2alpha; CBF2alpha; PEBP2aB; EVI-1
Background

Gene Name: RUNX1/RUNX2/RUNX3

NCBI Gene Entry: 861/864/860

UniProt Entry: Q01196/Q13761/Q13950
Application Information

Molecular Weight: Predicted, 49 kDa, observed, 51 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB4800

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human RUNX1/RUNX2/RUNX3
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of RUNX1/RUNX2/RUNX3 expression in HT-1080 cells using RUNX1/RUNX2/RUNX3 antibody (Cat#61851, 1:2,000). Green, isotype control; red, RUNX1/RUNX2/RUNX3.
  • Immunocytochemical staining of HT-1080 cells with anti-RUNX1/RUNX2/RUNX3 antibody (Cat #61851, 1:1,000) . Nuclei were stained blue with DAPI; RUNX1/RUNX2/RUNX3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-RUNX1/RUNX2/RUNX3 antibody (Cat#61851). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RUNX1/RUNX2/RUNX3 antibody (Cat#61851, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-RUNX1/RUNX2/RUNX3 antibody (Cat#61851). RUNX1/RUNX2/RUNX3 expression in wild type (WT) and RUNX1/RUNX2/RUNX3 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-RUNX1/RUNX2/RUNX3 antibody (Cat#61851, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
  • 基因名：
    RUNX1/RUNX2/RUNX3
  • 货号：
    61851
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of RUNX1/RUNX2/RUNX3 expression in HT-1080 cells using RUNX1/RUNX2/RUNX3 antibody (Cat#61851, 1:2,000). Green, isotype control; red, RUNX1/RUNX2/RUNX3.
Immunocytochemical staining of HT-1080 cells with anti-RUNX1/RUNX2/RUNX3 antibody (Cat #61851, 1:1,000) . Nuclei were stained blue with DAPI; RUNX1/RUNX2/RUNX3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-RUNX1/RUNX2/RUNX3 antibody (Cat#61851). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RUNX1/RUNX2/RUNX3 antibody (Cat#61851, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-RUNX1/RUNX2/RUNX3 antibody (Cat#61851). RUNX1/RUNX2/RUNX3 expression in wild type (WT) and RUNX1/RUNX2/RUNX3 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-RUNX1/RUNX2/RUNX3 antibody (Cat#61851, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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