Flow cytometric analysis of Dipeptidyl peptidase 9 expression in HepG2 cells using anti-Dipeptidyl peptidase 9 antibody (Cat#61855, 1:1,000). Green, isotype control; red, Dipeptidyl peptidase 9.
Immunocytochemical staining of HepG2 cells with anti-Dipeptidyl peptidase 9 antibody (Cat#61855, 1:1,000). Nuclei were stained blue with DAPI; Dipeptidyl peptidase 9 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-dipeptidyl peptidase 9 antibody (Cat#61855). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-dipeptidyl peptidase 9 antibody (Cat#61855, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-dipeptidyl peptidase 9 antibody (Cat#61855). Dipeptidyl peptidase 9 expression in wild type (WT) and dipeptidyl peptidase 9(DPP9) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-dipeptidyl peptidase 9 antibody (Cat#61855, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQTM ECL Substrate Kit (Cat#226).
Validation of Dipeptidyl peptidase 9 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Dipeptidyl peptidase 9 antibody (Cat#61855, 1:2,000) and analyzed using BD flow cytometer.
