Flow cytometric analysis of Epsin 2 expression in HeLa cells using anti-Epsin 2 antibody (Cat#61860, 1:1,000). Green, isotype control; red, Epsin 2.
Immunocytochemical staining of Hela cells with anti-Epsin 2 antibody (Cat#61860, 1:1,000). Nuclei were stained blue with DAPI; Epsin 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-epsin 2 antibody (Cat#61860). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-epsin 2 antibody (Cat#61860, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-epsin 2 antibody (Cat#61860). Epsin 2 expression in wild type (WT) and epsin 2 (EPN2) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-epsin 2 antibody (Cat#61860, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of Epsin 2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Epsin 2 antibody (Cat#61860, 1:2,000) and analyzed using BD flow cytometer.
