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KD-Validated Anti-ROS1 Mouse Monoclonal Antibody #61882

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Flow cytometric analysis of ROS1 expression in HAP-1 cells using anti-ROS1 antibody (Cat#61882, 1:2,000). Green, isotype control; red, ROS1.

Western blotting analysis using anti-ROS1 antibody (Cat#61882). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ROS1 antibody (Cat#61882, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Western blotting analysis using anti-ROS1 antibody (Cat#61882). ROS1 expression in wild type (WT) and ROS1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-ROS1 antibody (Cat#61882, 1:500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Validation of ROS1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-ROS1 antibody (Cat#61882, 1:2,000) and analyzed using BD flow cytometer.

基因名：

ROS1
货号：

61882
应用：

WB, FCM
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information

Product Name	KD-Validated Anti-ROS1 Mouse Monoclonal Antibody
Aliases	ROS1; ROS Proto-Oncogene 1, Receptor Tyrosine Kinase; MCF3; ROS; C-Ros-1; V-Ros Avian UR2 Sarcoma Virus Oncogene Homolog 1; C-Ros Oncogene 1 , Receptor Tyrosine Kinase; Proto-Oncogene Tyrosine-Protein Kinase ROS; Proto-Oncogene C-Ros-1; V-Ros UR2 Sarcoma Virus Oncogene Homolog 1 (Avian); ROS Proto-Oncogene 1 , Receptor Tyrosine Kinase; Transmembrane Tyrosine-Specific Protein Kinase; Receptor Tyrosine Kinase C-Ros Oncogene 1; C-Ros Receptor Tyrosine Kinase; Proto-Oncogene C-Ros; EC 2.7.10.1
Background	Gene Name: ROS1 NCBI Gene Entry: 6098 UniProt Entry: P08922
Application Information	Molecular Weight: Predicted, 264 kDa; observed, 264 kDa Clonality: Mouse monoclonal antibody Clone ID: 25GB4065 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen	Recombinant protein of human ROS1
Isotype	Mouse IgG2a
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:400-1:2,000 Flow Cytometry (FCM): 1:2,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of ROS1 expression in HAP-1 cells using anti-ROS1 antibody (Cat#61882, 1:2,000). Green, isotype control; red, ROS1.
Western blotting analysis using anti-ROS1 antibody (Cat#61882). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ROS1 antibody (Cat#61882, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-ROS1 antibody (Cat#61882). ROS1 expression in wild type (WT) and ROS1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-ROS1 antibody (Cat#61882, 1:500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of ROS1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-ROS1 antibody (Cat#61882, 1:2,000) and analyzed using BD flow cytometer.

Citation(0)

基因名：

ROS1
货号：

61882
应用：

WB, FCM
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

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