Western blotting analysis using anti-HSPH1 antibody (Cat#61898). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HSPH1 antibody (Cat#61898, 1:2,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-HSPH1 antibody (Cat#61898). HSPH1 expression in wild-type (WT) and HSPH1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-HSPH1 antibody (Cat#61898, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
HSPH1; Heat Shock Protein Family H (Hsp110) Member 1; KIAA0201; NY-CO-25; HSP105B; HSP105A; Heat Shock Protein Family H Member 1; Heat Shock 105kDa/110kDa Protein 1; Heat Shock Protein 105 KDa; Antigen NY-CO-25; HSP105; Heat Shock 110 KDa Protein; HSP110
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:400-1:2,000
Note
This product is for research use only.
Picture
Western blotting analysis using anti-HSPH1 antibody (Cat#61898). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HSPH1 antibody (Cat#61898, 1:2,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-HSPH1 antibody (Cat#61898). HSPH1 expression in wild-type (WT) and HSPH1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-HSPH1 antibody (Cat#61898, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-HSPH1 antibody (Cat#61898). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HSPH1 antibody (Cat#61898, 1:2,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-HSPH1 antibody (Cat#61898). HSPH1 expression in wild-type (WT) and HSPH1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-HSPH1 antibody (Cat#61898, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
