Flow cytometric analysis of DCP1A expression in HT-1080 cells using DCP1A antibody (Cat#61925, 1:2,000). Green, isotype control; red, DCP1A.
Immunocytochemical staining of HT-1080 cells with anti-DCP1A antibody (Cat #61925, 1:1,000) . Nuclei were stained blue with DAPI; DCP1A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-DCP1A antibody (Cat#61925). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-DCP1A antibody (Cat#61925, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-DCP1A antibody (Cat#61925). DCP1A expression in wild type (WT) and DCP1A shRNA knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-DCP1A antibody (Cat#61925, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
