Flow cytometric analysis of GTF2I expression in HAP-1 cells using anti-GTF2I antibody (Cat#62090, 1:2,000). Green, isotype control; red, GTF2I .
Immunocytochemical staining of HAP1 cells with anti-GTF2I antibody (Cat #62090, 1:1,000) . Nuclei were stained blue with DAPI;GTF2I was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-GTF2I antibody (Cat#62090). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-GTF2I antibody (Cat#62090, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-GTF2I antibody (Cat#62090). GTF2I expression in wild type (WT) and GTF2I shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-GTF2I antibody (Cat#62090, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
