Flow cytometric analysis of F11 receptor expression in HAP-1 cells using F11 receptor antibody (Cat#62116, 1:2,000). Green, isotype control; red, F11 receptor.
Immunocytochemical staining of HAP-1 cells with F11 receptor antibody (Cat#62116, 1:1,000). Nuclei were stained blue with DAPI; F11 receptor was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-F11 receptor antibody (Cat#62116). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-F11 receptor antibody (Cat#62116, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-F11 receptor antibody (Cat#62116). F11 receptor expression in wild type (WT) and F11 receptor shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-F11 receptor antibody (Cat#62116, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
