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KD-Validated Anti-Poly(A) Binding Protein Nuclear 1 Rabbit Monoclonal Antibody #62337

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Flow cytometric analysis of PABPN1 expression in HepG2 cells using PABPN1 antibody (Cat#62337, 1:2,000). Green, isotype control; red, PABPN1.
Immunocytochemical staining of Hela cells with anti-PABPN1 antibody (Cat#62337, 1:1,000). Nuclei were stained blue with DAPI; PABPN1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-PABPN1 antibody (Cat#62337). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PABPN1 antibody (Cat#62337, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-PABPN1 antibody (Cat#62337). PABPN1 expression in wild type (WT) and PABPN1 shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-PABPN1 antibody (Cat#62337, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HT1080 cells using anti-PABPN1 antibody (Cat#62337, 1:1,000), Top panel: wild-type (WT); Bottom panal: PABPN1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; PABPN1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
  • 基因名：
    PABPN1
  • 货号：
    62337
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-Poly(A) Binding Protein Nuclear 1 Rabbit Monoclonal Antibody
Aliases Poly(A) Binding Protein Nuclear 1; PAB2 ; PABP2; Polyadenylate-Binding Protein 2; PABP-2; PABII; OPMD; Polyadenylate-Binding Nuclear Protein 1; Poly(A)-Binding Protein, Nuclear 1; Poly(A) Binding Protein, Nuclear 1; Nuclear Poly(A)-Binding Protein 1; Poly(A) Binding Protein II; Poly(A)-Binding Protein II; Poly(A) Binding Protein 2; Poly(A)-Binding Protein 2
Background

Gene Name: PABPN1

NCBI Gene Entry: 8106

UniProt Entry: Q86U42
Application Information

Molecular Weight: Predicted, 33 kDa, observed, 49 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB365

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human PABPN1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of PABPN1 expression in HepG2 cells using PABPN1 antibody (Cat#62337, 1:2,000). Green, isotype control; red, PABPN1.
  • Immunocytochemical staining of Hela cells with anti-PABPN1 antibody (Cat#62337, 1:1,000). Nuclei were stained blue with DAPI; PABPN1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
  • Western blotting analysis using anti-PABPN1 antibody (Cat#62337). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PABPN1 antibody (Cat#62337, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-PABPN1 antibody (Cat#62337). PABPN1 expression in wild type (WT) and PABPN1 shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-PABPN1 antibody (Cat#62337, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HT1080 cells using anti-PABPN1 antibody (Cat#62337, 1:1,000), Top panel: wild-type (WT); Bottom panal: PABPN1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; PABPN1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
Citation(0)
  • 基因名：
    PABPN1
  • 货号：
    62337
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of PABPN1 expression in HepG2 cells using PABPN1 antibody (Cat#62337, 1:2,000). Green, isotype control; red, PABPN1.
Immunocytochemical staining of Hela cells with anti-PABPN1 antibody (Cat#62337, 1:1,000). Nuclei were stained blue with DAPI; PABPN1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-PABPN1 antibody (Cat#62337). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PABPN1 antibody (Cat#62337, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-PABPN1 antibody (Cat#62337). PABPN1 expression in wild type (WT) and PABPN1 shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-PABPN1 antibody (Cat#62337, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HT1080 cells using anti-PABPN1 antibody (Cat#62337, 1:1,000), Top panel: wild-type (WT); Bottom panal: PABPN1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; PABPN1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
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