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KD-Validated Anti-Mov10 RNA Helicase Rabbit Monoclonal Antibody #62382

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Flow cytometric analysis of MOV10 expression in Hela cells using anti-MOV10 antibody (Cat#62382, 1:2,000). Green, isotype control; red, MOV10.
Immunocytochemical staining of HeLa cells with anti-MOV10 antibody (Cat#62382, 1:1,000). Nuclei were stained blue with DAPI; MOV10 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-MOV10 antibody (Cat#62382). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MOV10 antibody (Cat#62382, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Mov10 antibody (Cat#62382). Mov10 expression in wild type (WT) and Mov10 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Mov10 antibody (Cat#62382, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名:
    MOV10
  • 货号:
    62382
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 650.00,¥ 1,350.00,¥ 2,500.00
Information
Product Name KD-Validated Anti-Mov10 RNA Helicase Rabbit Monoclonal Antibody
Aliases Mov10 RNA Helicase; Mov10 RISC Complex RNA Helicase; FSAP113; Gb110; Functional Spliceosome-Associated Protein 113; Moloney Leukemia Virus 10 Protein; Armitage Homolog; Helicase MOV-10; MGC2948; Mov10, Moloney Leukemia Virus 10, Homolog (Mouse); Mov10 (Moloney Leukemia Virus 10, Mouse) Homolog; Mov10, Moloney Leukemia Virus 10, Homolog; Putative Helicase MOV-10; EC 3.6.4.13; KIAA1631; EC 3.6.1
Background

Gene Name: MOV10

NCBI Gene Entry: 4343

UniProt Entry: Q9HCE1

Application Information

Molecular Weight: Predicted, 114 kDa, observed, 114 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB575

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human Mov10
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of MOV10 expression in Hela cells using anti-MOV10 antibody (Cat#62382, 1:2,000). Green, isotype control; red, MOV10.
  • Immunocytochemical staining of HeLa cells with anti-MOV10 antibody (Cat#62382, 1:1,000). Nuclei were stained blue with DAPI; MOV10 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
  • Western blotting analysis using anti-MOV10 antibody (Cat#62382). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MOV10 antibody (Cat#62382, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-Mov10 antibody (Cat#62382). Mov10 expression in wild type (WT) and Mov10 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Mov10 antibody (Cat#62382, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
Flow cytometric analysis of MOV10 expression in Hela cells using anti-MOV10 antibody (Cat#62382, 1:2,000). Green, isotype control; red, MOV10.
Immunocytochemical staining of HeLa cells with anti-MOV10 antibody (Cat#62382, 1:1,000). Nuclei were stained blue with DAPI; MOV10 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-MOV10 antibody (Cat#62382). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MOV10 antibody (Cat#62382, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Mov10 antibody (Cat#62382). Mov10 expression in wild type (WT) and Mov10 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Mov10 antibody (Cat#62382, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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