Flow cytometric analysis of Olfactomedin 4 expression in C2C12 cells using Olfactomedin 4 antibody (Cat#62403, 1:2,000). Green, isotype control; red, Olfactomedin 4.
Immunocytochemical staining of C2C12 cells with anti-Olfactomedin 4 antibody (Cat#62403, 1:1,000) . Nuclei were stained blue with DAPI; Olfactomedin 4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-Olfactomedin 4 antibody (Cat#62403). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Olfactomedin 4 antibody (Cat#62403, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Olfactomedin 4 antibody (Cat#62403). Olfactomedin 4 expression in wild type (WT) and Olfactomedin 4 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Olfactomedin 4 antibody (Cat#62403, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).