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KD-Validated Anti-PCBD1 Rabbit Monoclonal Antibody #62427

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Immunocytochemical staining of HepG2 cells with anti-PCBD1 antibody (Cat#62427, 1:1,000). Nuclei were stained blue with DAPI; PCBD1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-PCBD1 antibody (Cat#62427). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PCBD1 antibody (Cat#62427, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-PCBD1 antibody (Cat#62427). PCBD1 expression in wild-type (WT) and PCBD1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-PCBD1 antibody (Cat#62427, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名:
    PCBD1
  • 货号:
    62427
  • 应用:
    WB, IC
  • 物种反应性:
    H
规格:
20 μL,50 μL,100 μL
价格:
¥ 650.00,¥ 1,350.00,¥ 2,500.00
Information
Product Name KD-Validated Anti-PCBD1 Rabbit Monoclonal Antibody
Aliases Pterin-4 Alpha-Carbinolamine Dehydratase 1; PCD; DCOH; PCBD; 6-Pyruvoyl-Tetrahydropterin Synthase/Dimerization Cofactor Of Hepatocyte Nuclear Factor 1 Alpha (TCF1); Pterin-4 Alpha-Carbinolamine Dehydratase/Dimerization Cofactor Of Hepatocyte Nuclear Factor 1 Alpha; Phenylalanine Hydroxylase-Stimulating Protein; 4-Alpha-Hydroxy-Tetrahydropterin Dehydratase; Pterin-4-Alpha-Carbinolamine Dehydratase; Dimerizing Cofactor For HNF1; EC 4.2.1.96; PHS; Pterin-4 Alpha-Carbinolamine Dehydratase/Dimerization Cofactor Of Hepatocyte Nuclear Factor 1 Alpha (TCF1); Pterin-4a-Carbinolamine Dehydratase (Dimerization Cofactor Of Hepatic Nuclear Factor 1-Alpha); Dimerization Cofactor Of Hepatocyte Nuclear Factor 1-Alpha; Pterin-4-Alpha Carbinolamine Dehydratase; Pterin Carbinolamine Dehydratase; Dimerization Cofactor Of HNF1; DCoH
Background

Gene Name: PCBD1

NCBI Gene Entry: 5092

UniProt Entry: P61457

Application Information

Molecular Weight: Predicted, 12 kDa, observed, 12 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB785

Species Reactivity: Human

Applications Tested: Western blotting (WB), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human PCBD1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Immunocytochemical staining of HepG2 cells with anti-PCBD1 antibody (Cat#62427, 1:1,000). Nuclei were stained blue with DAPI; PCBD1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
  • Western blotting analysis using anti-PCBD1 antibody (Cat#62427). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PCBD1 antibody (Cat#62427, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-PCBD1 antibody (Cat#62427). PCBD1 expression in wild-type (WT) and PCBD1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-PCBD1 antibody (Cat#62427, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
Immunocytochemical staining of HepG2 cells with anti-PCBD1 antibody (Cat#62427, 1:1,000). Nuclei were stained blue with DAPI; PCBD1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-PCBD1 antibody (Cat#62427). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PCBD1 antibody (Cat#62427, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-PCBD1 antibody (Cat#62427). PCBD1 expression in wild-type (WT) and PCBD1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-PCBD1 antibody (Cat#62427, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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