Flow cytometric analysis of Protein arginine methyltransferase 5 expression in HeLa cells using Protein arginine methyltransferase 5 antibody (Cat#62439, 1:2,000). Green, isotype control; red, Protein arginine methyltransferase 5.
Immunocytochemical staining of Hela cells with anti-Protein arginine methyltransferase 5 antibody (Cat#62439, 1:1,000). Nuclei were stained blue with DAPI; Protein arginine methyltransferase 5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Protein arginine methyltransferase 5 antibody (Cat#62439). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Protein arginine methyltransferase 5 antibody (Cat#62439, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-protein arginine methyltransferase 5 antibody (Cat#62439). Protein arginine methyltransferase 5 expression in wild-type (WT) and protein arginine methyltransferase 5 (PRMT5) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-protein arginine methyltransferase 5 antibody (Cat#62439, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
