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KD-Validated Anti-PERP Rabbit Monoclonal Antibody #62608

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Flow cytometric analysis of PERP expression in Hela cells using anti-PERP antibody (Cat#62608, 1:2,000). Green, isotype control; red, PERP.
Western blotting analysis using anti-PERP antibody (Cat#62608). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PERP antibody (Cat#62608, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-PERP antibody (Cat#62608). PERP expression in wild type (WT) and PERP shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-PERP antibody (Cat#62608, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of PERP knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-PERP antibody (Cat#62608, 1:2,000) and analyzed using BD flow cytometer.
  • 基因名:
    PERP
  • 货号:
    62608
  • 应用:
    WB, FCM
  • 物种反应性:
    H
规格:
20 μL,50 μL,100 μL
价格:
¥ 650.00,¥ 1,350.00,¥ 2,500.00
Information
Product Name KD-Validated Anti-PERP Rabbit Monoclonal Antibody
Aliases P53 Apoptosis Effector Related To PMP22; KRTCAP1; PIGPC1; KCP1; THW; DJ496H19.1; P53 Apoptosis Effector Related To PMP-22; Keratinocyte-Associated Protein 1; PERP, TP53 Apoptosis Effector; P53-Induced Protein PIGPC1; Transmembrane Protein THW; KCP-1; Keratinocytes Associated Protein 1; Keratinocyte Associated Protein 1; 1110017A08Rik; EKVP7; OLMS2
Background

Gene Name: PERP

NCBI Gene Entry: 64065

UniProt Entry: Q96FX8

Application Information

Molecular Weight: Predicted, 21 kDa, observed, 21 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB1630

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen A synthesized peptide derived from human PERP
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of PERP expression in Hela cells using anti-PERP antibody (Cat#62608, 1:2,000). Green, isotype control; red, PERP.
  • Western blotting analysis using anti-PERP antibody (Cat#62608). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PERP antibody (Cat#62608, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-PERP antibody (Cat#62608). PERP expression in wild type (WT) and PERP shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-PERP antibody (Cat#62608, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Validation of PERP knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-PERP antibody (Cat#62608, 1:2,000) and analyzed using BD flow cytometer.
Citation(0)
Flow cytometric analysis of PERP expression in Hela cells using anti-PERP antibody (Cat#62608, 1:2,000). Green, isotype control; red, PERP.
Western blotting analysis using anti-PERP antibody (Cat#62608). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PERP antibody (Cat#62608, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-PERP antibody (Cat#62608). PERP expression in wild type (WT) and PERP shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-PERP antibody (Cat#62608, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of PERP knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-PERP antibody (Cat#62608, 1:2,000) and analyzed using BD flow cytometer.
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