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KD-Validated Anti-TAF15 Rabbit Monoclonal Antibody #62622

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Flow cytometric analysis of TAF15 expression in HepG2 cells using anti-TAF15 antibody (Cat#62622, 1:2,000). Green, isotype control; red, TAF15.
Immunocytochemical staining of HepG2 cells with anti-TAF15 antibody (Cat#62622, 1:1,000). Nuclei were stained blue with DAPI; TAF15 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-TAF15 antibody (Cat#62622). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-TAF15 antibody (Cat#62622, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-TAF15 antibody (Cat#62622). TAF15 expression in wild type (WT) and TAF15 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-TAF15 antibody (Cat#62622, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名：
    TAF15
  • 货号：
    62622
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-TAF15 Rabbit Monoclonal Antibody
Aliases TAF15; TATA-Box Binding Protein Associated Factor 15; RBP56; TAF2N; Npl3; TATA Box Binding Protein (TBP)-Associated Factor, RNA Polymerase II, N, 68kD (RNA-Binding Protein 56); TAF15 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 68kDa; TATA-Binding Protein-Associated Factor 2N; HTAFII68; TAFII68; TATA Box-Binding Protein-Associated Factor 2N (RNA-Binding Protein 56); 68 KDa TATA-Binding Protein-Associated Factor; TBP-Associated Factor 15; RNA-Binding Protein 56; RBP56/CSMF Fusion; TAF(II)68
Background

Gene Name: TAF15

NCBI Gene Entry: 8148

UniProt Entry: Q92804
Application Information

Molecular Weight: Predicted, 62 kDa, observed, 62 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB1830

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human TAF15
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of TAF15 expression in HepG2 cells using anti-TAF15 antibody (Cat#62622, 1:2,000). Green, isotype control; red, TAF15.
  • Immunocytochemical staining of HepG2 cells with anti-TAF15 antibody (Cat#62622, 1:1,000). Nuclei were stained blue with DAPI; TAF15 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
  • Western blotting analysis using anti-TAF15 antibody (Cat#62622). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-TAF15 antibody (Cat#62622, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-TAF15 antibody (Cat#62622). TAF15 expression in wild type (WT) and TAF15 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-TAF15 antibody (Cat#62622, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
  • 基因名：
    TAF15
  • 货号：
    62622
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of TAF15 expression in HepG2 cells using anti-TAF15 antibody (Cat#62622, 1:2,000). Green, isotype control; red, TAF15.
Immunocytochemical staining of HepG2 cells with anti-TAF15 antibody (Cat#62622, 1:1,000). Nuclei were stained blue with DAPI; TAF15 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-TAF15 antibody (Cat#62622). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-TAF15 antibody (Cat#62622, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-TAF15 antibody (Cat#62622). TAF15 expression in wild type (WT) and TAF15 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-TAF15 antibody (Cat#62622, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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