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KD-Validated Anti-U2AF2 Rabbit Monoclonal Antibody #62670

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Flow cytometric analysis of U2AF2 expression in HepG2 cells using anti-U2AF2 antibody (Cat#62670, 1:2,000). Green, isotype control; red, U2AF2.
Immunocytochemical staining of HepG2 cells with anti-U2AF2 antibody (Cat#62670, 1:1,000). Nuclei were stained blue with DAPI; U2AF2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-U2AF2 antibody (Cat#62670). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-U2AF2 antibody (Cat#62670, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-U2AF2 antibody (Cat#62670). U2AF2 expression in wild-type (WT) and U2AF2 shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-U2AF2 antibody (Cat#62670, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名：
    U2AF2
  • 货号：
    62670
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-U2AF2 Rabbit Monoclonal Antibody
Aliases U2AF2; U2 Small Nuclear RNA Auxiliary Factor 2; U2AF65; U2 SnRNP Auxiliary Factor Large Subunit; U2 Small Nuclear Ribonucleoprotein Auxiliary Factor (65kD); U2 (RNU2) Small Nuclear RNA Auxiliary Factor 2; Splicing Factor U2AF 65 KDa Subunit; U2 Auxiliary Factor 65 KDa Subunit; HU2AF65; Splicing Factor U2AF 65 KD Subunit; HU2AF(65); DEVDFB
Background

Gene Name: U2AF2

NCBI Gene Entry: 11338

UniProt Entry: P26368
Application Information

Molecular Weight: Predicted, 54 kDa, observed, 54 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB2465

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human U2AF65
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of U2AF2 expression in HepG2 cells using anti-U2AF2 antibody (Cat#62670, 1:2,000). Green, isotype control; red, U2AF2.
  • Immunocytochemical staining of HepG2 cells with anti-U2AF2 antibody (Cat#62670, 1:1,000). Nuclei were stained blue with DAPI; U2AF2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
  • Western blotting analysis using anti-U2AF2 antibody (Cat#62670). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-U2AF2 antibody (Cat#62670, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-U2AF2 antibody (Cat#62670). U2AF2 expression in wild-type (WT) and U2AF2 shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-U2AF2 antibody (Cat#62670, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
  • 基因名：
    U2AF2
  • 货号：
    62670
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of U2AF2 expression in HepG2 cells using anti-U2AF2 antibody (Cat#62670, 1:2,000). Green, isotype control; red, U2AF2.
Immunocytochemical staining of HepG2 cells with anti-U2AF2 antibody (Cat#62670, 1:1,000). Nuclei were stained blue with DAPI; U2AF2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-U2AF2 antibody (Cat#62670). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-U2AF2 antibody (Cat#62670, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-U2AF2 antibody (Cat#62670). U2AF2 expression in wild-type (WT) and U2AF2 shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-U2AF2 antibody (Cat#62670, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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