Flow cytometric analysis of Sorting nexin 5 expression in HAP-1 cells using anti-Sorting nexin 5 antibody (Cat#62718, 1:2,000). Green, isotype control; red, Sorting nexin 5.
Immunocytochemical staining of HAP1 cells with anti-Sorting nexin 5 antibody (Cat#62718, 1:1,000) . Nuclei were stained blue with DAPI;Sorting nexin 5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Low. Scale bar, 20 μm.
Western blotting analysis using anti-Sorting nexin 5 antibody (Cat#62718). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Sorting nexin 5 antibody (Cat#62718, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Sorting nexin 5 antibody (Cat#62718). Sorting nexin 5 expression in wild type (WT) and Sorting nexin 5 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Sorting nexin 5 antibody (Cat#62718, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).