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KD-Validated Anti-TNF Receptor Associated Protein 1 Rabbit Monoclonal Antibody #62739

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Flow cytometric analysis of TNF receptor associated protein 1 expression in HepG2 cells using anti-TNF receptor associated protein 1 antibody (Cat#62739, 1:2,000). Green, isotype control; red, TNF receptor associated protein 1.
Immunocytochemical staining of HepG2 cells with anti-TNF receptor associated protein 1 antibody (Cat#62739, 1:1,000). Nuclei were stained blue with DAPI; TNF receptor associated protein 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-TNF receptor associated protein 1 antibody (Cat#62739). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-TNF receptor associated protein 1 antibody (Cat#62739, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-TNF receptor associated protein 1 antibody (Cat#62739). TNF receptor associated protein 1 expression in wild type (WT) and TNF receptor associated protein 1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-TNF receptor associated protein 1 antibody (Cat#62739, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of TNF receptor associated protein 1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-TNF receptor associated protein 1 antibody (Cat#62739, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of HeLa cells using anti-TNF receptor associated protein 1 antibody (Cat#62739, 1:1,000), Top panel: wild-type (WT); Bottom panal: TNF receptor associated protein 1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; TNF receptor associated protein 1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
  • 基因名：
    TRAP1
  • 货号：
    62739
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-TNF Receptor Associated Protein 1 Rabbit Monoclonal Antibody
Aliases TRAP1; TNF Receptor Associated Protein 1; HSP75; HSP90L; Tumor Necrosis Factor Type 1 Receptor-Associated Protein; Heat Shock Protein 75 KDa, Mitochondrial; TNFR-Associated Protein 1; HSP 75; TRAP-1; TNF Receptor-Associated Protein 1; Testicular Tissue Protein Li 209
Background

Gene Name: TRAP1

NCBI Gene Entry: 10131

UniProt Entry: Q12931
Application Information

Molecular Weight: Predicted, 80 kDa, observed, 75 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB2560

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human TRAP1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of TNF receptor associated protein 1 expression in HepG2 cells using anti-TNF receptor associated protein 1 antibody (Cat#62739, 1:2,000). Green, isotype control; red, TNF receptor associated protein 1.
  • Immunocytochemical staining of HepG2 cells with anti-TNF receptor associated protein 1 antibody (Cat#62739, 1:1,000). Nuclei were stained blue with DAPI; TNF receptor associated protein 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
  • Western blotting analysis using anti-TNF receptor associated protein 1 antibody (Cat#62739). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-TNF receptor associated protein 1 antibody (Cat#62739, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-TNF receptor associated protein 1 antibody (Cat#62739). TNF receptor associated protein 1 expression in wild type (WT) and TNF receptor associated protein 1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-TNF receptor associated protein 1 antibody (Cat#62739, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Validation of TNF receptor associated protein 1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-TNF receptor associated protein 1 antibody (Cat#62739, 1:2,000) and analyzed using BD flow cytometer.
  • Immunocytochemical staining of HeLa cells using anti-TNF receptor associated protein 1 antibody (Cat#62739, 1:1,000), Top panel: wild-type (WT); Bottom panal: TNF receptor associated protein 1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; TNF receptor associated protein 1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
Citation(0)
  • 基因名：
    TRAP1
  • 货号：
    62739
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of TNF receptor associated protein 1 expression in HepG2 cells using anti-TNF receptor associated protein 1 antibody (Cat#62739, 1:2,000). Green, isotype control; red, TNF receptor associated protein 1.
Immunocytochemical staining of HepG2 cells with anti-TNF receptor associated protein 1 antibody (Cat#62739, 1:1,000). Nuclei were stained blue with DAPI; TNF receptor associated protein 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-TNF receptor associated protein 1 antibody (Cat#62739). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-TNF receptor associated protein 1 antibody (Cat#62739, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-TNF receptor associated protein 1 antibody (Cat#62739). TNF receptor associated protein 1 expression in wild type (WT) and TNF receptor associated protein 1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-TNF receptor associated protein 1 antibody (Cat#62739, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of TNF receptor associated protein 1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-TNF receptor associated protein 1 antibody (Cat#62739, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of HeLa cells using anti-TNF receptor associated protein 1 antibody (Cat#62739, 1:1,000), Top panel: wild-type (WT); Bottom panal: TNF receptor associated protein 1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; TNF receptor associated protein 1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
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