Immunocytochemical staining of HT-1080 cells with anti-Synaptosome associated protein 25 antibody(Cat#62795, 1:1,000) . Nuclei were stained blue with DAPI; Synaptosome associated protein 25 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-Synaptosome associated protein 25 antibody (Cat#62795). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Synaptosome associated protein 25 antibody (Cat#62795, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Synaptosome associated protein 25 antibody (Cat#62795). Synaptosome associated protein 25 expression in wild type (WT) and Synaptosome associated protein 25 shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Synaptosome associated protein 25 antibody (Cat#62795, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
