Flow cytometric analysis of Sonic Hedgehog expression in HepG2 cells using anti-Sonic Hedgehog antibody (Cat#62804, 1:2,000). Green, isotype control; red, Sonic Hedgehog.
Immunocytochemical staining of HepG2 cells with anti-Sonic Hedgehog antibody (Cat#62804, 1:1,000). Nuclei were stained blue with DAPI; Sonic Hedgehog was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-sonic hedgehog antibody (Cat#62804). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-sonic hedgehog antibody (Cat#62804, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-sonic hedgehog antibody (Cat#62804). sonic hedgehog expression in wild-type (WT) and SHH shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-sonic hedgehog antibody (Cat#62804, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). SHH, sonic hedgehog signaling molecule.
