KD-Validated Anti-BUB1B Rabbit Polyclonal Antibody - 产品中心

KD-Validated Anti-BUB1B Rabbit Polyclonal Antibody #62829

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Western blotting analysis using anti-BUB1B antibody (Cat#62829). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-BUB1B antibody (Cat#62829, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Western blotting analysis using anti-BUB1B kinase antibody (Cat#62829). BUB1B kinase expression in wild type (WT) and BUB1B shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-BUB1B kinase antibody (Cat#62829, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

BUB1B
货号：

62829
应用：

WB
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

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Information

Product Name	KD-Validated Anti-BUB1B Rabbit Polyclonal Antibody
Aliases	BUB1B; BUB1 Mitotic Checkpoint Serine/Threonine Kinase B; BUBR1; MAD3L; SSK1; Bub1A; Mitotic Checkpoint Serine/Threonine-Protein Kinase BUB1 Beta; MAD3/BUB1-Related Protein Kinase; Mitotic Checkpoint Kinase MAD3L; HBUBR1; Budding Uninhibited By Benzimidazoles 1 (Yeast Homolog), Beta; Budding Uninhibited By Benzimidazoles 1 Homolog Beta (Yeast); Budding Uninhibited By Benzimidazoles 1 Homolog Beta; BUB1B, Mitotic Checkpoint Serine/Threonine Kinase; Protein SSK1; EC 2.7.11.1; BUB1beta; MVA1
Background	Gene Name: BUB1B NCBI Gene Entry: 701 UniProt Entry: O60566
Application Information	Molecular Weight: Predicted, 120 kDa, observed, 130 kDa Clonality: Rabbit polyclonal antibody Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB)
Immunogen	A synthesized peptide derived from human BUB1B
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:2,500-1:5,000
Note	This product is for research use only.

Picture

Western blotting analysis using anti-BUB1B antibody (Cat#62829). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-BUB1B antibody (Cat#62829, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-BUB1B kinase antibody (Cat#62829). BUB1B kinase expression in wild type (WT) and BUB1B shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-BUB1B kinase antibody (Cat#62829, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

BUB1B
货号：

62829
应用：

WB
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

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