Immunocytochemical staining of HepG2 cells with anti-Adenylate kinase 1 antibody(Cat#62872, 1:1,000) . Nuclei were stained blue with DAPI; Adenylate kinase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-Adenylate kinase 1 antibody (Cat#62872). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Adenylate kinase 1 antibody (Cat#62872, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-adenylate kinase 1 antibody (Cat#62872). Adenylate kinase 1 expression in wild-type (WT) and adenylate kinase 1 (AK1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-adenylate kinase 1 antibody (Cat#62872, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with anti-Adenylate kinase 1 antibody(Cat#62872, 1:1,000) . Nuclei were stained blue with DAPI; Adenylate kinase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
