Flow cytometric analysis of Mitogen-activated protein kinase 1 expression in C2C12 cells using anti-Mitogen-activated protein kinase 1 antibody (Cat#62904, 1:2,000). Green, isotype control; red, Mitogen-activated protein kinase 1.
Immunocytochemical staining of C2C12 cells with anti-Mitogen-activated protein kinase 1 antibody (Cat#62904, 1:1,000) . Nuclei were stained blue with DAPI; Mitogen-activated protein kinase 1 was stained magenta with Alexa Fluor® 647. Images were taken using leica stellaris 5. Protein abundance based on laser intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Mitogen-activated protein kinase 1 antibody (Cat#62904). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Mitogen-activated protein kinase 1 antibody (Cat#62904, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-mitogen-activated protein kinase 1 antibody (Cat#62904). Mitogen-activated protein kinase 1 expression in wild-type (WT) and mitogen-activated protein kinase 1 (MAPK1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-mitogen-activated protein kinase 1 antibody (Cat#62904, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of Mitogen-activated protein kinase 1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Mitogen-activated protein kinase 1 antibody (Cat#62904, 1:2,000) and analyzed using BD flow cytometer.
