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KD-Validated Anti-Serpin Family E Member 1 Rabbit Monoclonal Antibody #63098

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Flow cytometric analysis of Serpin family E member 1 expression in HT-1080 cells using anti-Serpin family E member 1 antibody (Cat#63098, 1:2,000). Green, isotype control; red, Serpin family E member 1.
Immunocytochemical staining of HT-1080 cells with anti-Serpin family E member 1 antibody (Cat#63098, 1:1,000) . Nuclei were stained blue with DAPI; Serpin family E member 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm.
Western blotting analysis using anti-serpin family E member 1 antibody (Cat#63098). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-serpin family E member 1 antibody (Cat#63098, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-serpin family E member 1 antibody (Cat#63098). Serpin family E member 1expression in wild-type (WT) and serpin family E member 1 (SERPINE1) shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-serpin family E member 1 antibody (Cat#63098, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名：
    SERPINE1
  • 货号：
    63098
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-Serpin Family E Member 1 Rabbit Monoclonal Antibody
Aliases SERPINE1; Serpin Family E Member 1; PAI 2; PLANH1; PAI1; Serine (Or Cysteine) Proteinase Inhibitor, Clade E (Nexin, Plasminogen Activator Inhibitor Type 1), Member 1; Endothelial Plasminogen Activator Inhibitor; Plasminogen Activator Inhibitor 1; Serpin E1; PAI-1; Serpin Peptidase Inhibitor, Clade E (Nexin, Plasminogen Activator Inhibitor Type 1), Member 1; Plasminogen Activator Inhibitor, Type I
Background

Gene Name: SERPINE1

NCBI Gene Entry: 5054

UniProt Entry: P05121
Application Information

Molecular Weight: Predicted, 45 kDa, observed, 45 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB4055

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human PAI1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Serpin family E member 1 expression in HT-1080 cells using anti-Serpin family E member 1 antibody (Cat#63098, 1:2,000). Green, isotype control; red, Serpin family E member 1.
  • Immunocytochemical staining of HT-1080 cells with anti-Serpin family E member 1 antibody (Cat#63098, 1:1,000) . Nuclei were stained blue with DAPI; Serpin family E member 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm.
  • Western blotting analysis using anti-serpin family E member 1 antibody (Cat#63098). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-serpin family E member 1 antibody (Cat#63098, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-serpin family E member 1 antibody (Cat#63098). Serpin family E member 1expression in wild-type (WT) and serpin family E member 1 (SERPINE1) shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-serpin family E member 1 antibody (Cat#63098, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
  • 基因名：
    SERPINE1
  • 货号：
    63098
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of Serpin family E member 1 expression in HT-1080 cells using anti-Serpin family E member 1 antibody (Cat#63098, 1:2,000). Green, isotype control; red, Serpin family E member 1.
Immunocytochemical staining of HT-1080 cells with anti-Serpin family E member 1 antibody (Cat#63098, 1:1,000) . Nuclei were stained blue with DAPI; Serpin family E member 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm.
Western blotting analysis using anti-serpin family E member 1 antibody (Cat#63098). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-serpin family E member 1 antibody (Cat#63098, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-serpin family E member 1 antibody (Cat#63098). Serpin family E member 1expression in wild-type (WT) and serpin family E member 1 (SERPINE1) shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-serpin family E member 1 antibody (Cat#63098, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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