Flow cytometric analysis of DNA methyltransferase 1 expression in HepG2 cells using anti-DNA methyltransferase 1 antibody (Cat#63144, 1:2,000). Green, isotype control; red, DNA methyltransferase 1.
Immunocytochemical staining of HepG2 cells with DNA methyltransferase 1 antibody (Cat#63144, 1:1,000). Nuclei were stained blue with DAPI; DNA methyltransferase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-DNA methyltransferase 1 antibody (Cat#63144). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-DNA methyltransferase 1 antibody (Cat#63144, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit(Cat#716).
Western blotting analysis using anti-DNA methyltransferase 1 antibody (Cat#63144). DNA methyltransferase 1 expression in wild type (WT) and DNA methyltransferase 1 (DNMT1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-DNA methyltransferase 1 antibody (Cat#63144, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of DNA methyltransferase 1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-DNA methyltransferase 1 antibody (Cat#63144, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of Hela cells using anti-DNA methyltransferase 1 antibody (Cat#63144, 1:1,000), Top panel: wild-type (WT); Bottom panal:DNA methyltransferase 1 shRNA knockdown (KD). Nuclei were stained blue with DAPI;DNA methyltransferase 1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.