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KD-Validated Anti-DNA Methyltransferase 1 Rabbit Monoclonal Antibody #63144

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Flow cytometric analysis of DNA methyltransferase 1 expression in HepG2 cells using anti-DNA methyltransferase 1 antibody (Cat#63144, 1:2,000). Green, isotype control; red, DNA methyltransferase 1.
Immunocytochemical staining of HepG2 cells with DNA methyltransferase 1 antibody (Cat#63144, 1:1,000). Nuclei were stained blue with DAPI; DNA methyltransferase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-DNA methyltransferase 1 antibody (Cat#63144). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-DNA methyltransferase 1 antibody (Cat#63144, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit(Cat#716).
Western blotting analysis using anti-DNA methyltransferase 1 antibody (Cat#63144). DNA methyltransferase 1 expression in wild type (WT) and DNA methyltransferase 1 (DNMT1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-DNA methyltransferase 1 antibody (Cat#63144, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of DNA methyltransferase 1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-DNA methyltransferase 1 antibody (Cat#63144, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of Hela cells using anti-DNA methyltransferase 1 antibody (Cat#63144, 1:1,000), Top panel: wild-type (WT); Bottom panal:DNA methyltransferase 1 shRNA knockdown (KD). Nuclei were stained blue with DAPI;DNA methyltransferase 1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
  • 基因名:
    DNMT1
  • 货号:
    63144
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 650.00,¥ 1,350.00,¥ 2,500.00
Information
Product Name KD-Validated Anti-DNA Methyltransferase 1 Rabbit Monoclonal Antibody
Aliases DNMT1; DNA Methyltransferase 1; CXXC9; MCMT; DNMT; DNA (Cytosine-5-)-Methyltransferase 1; DNA (Cytosine-5)-Methyltransferase 1; CXXC-Type Zinc Finger Protein 9; DNA Methyltransferase HsaI; DNA MTase HsaI; EC 2.1.1.37; M.HsaI; AIM; ADCADN; HSN1E; Dnmt1
Background

Gene Name: DNMT1

NCBI Gene Entry: 1786

UniProt Entry: P26358

Application Information

Molecular Weight: Predicted, 183 kDa, observed, 200 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB5135

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human Dnmt1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of DNA methyltransferase 1 expression in HepG2 cells using anti-DNA methyltransferase 1 antibody (Cat#63144, 1:2,000). Green, isotype control; red, DNA methyltransferase 1.
  • Immunocytochemical staining of HepG2 cells with DNA methyltransferase 1 antibody (Cat#63144, 1:1,000). Nuclei were stained blue with DAPI; DNA methyltransferase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
  • Western blotting analysis using anti-DNA methyltransferase 1 antibody (Cat#63144). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-DNA methyltransferase 1 antibody (Cat#63144, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit(Cat#716).
  • Western blotting analysis using anti-DNA methyltransferase 1 antibody (Cat#63144). DNA methyltransferase 1 expression in wild type (WT) and DNA methyltransferase 1 (DNMT1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-DNA methyltransferase 1 antibody (Cat#63144, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Validation of DNA methyltransferase 1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-DNA methyltransferase 1 antibody (Cat#63144, 1:2,000) and analyzed using BD flow cytometer.
  • Immunocytochemical staining of Hela cells using anti-DNA methyltransferase 1 antibody (Cat#63144, 1:1,000), Top panel: wild-type (WT); Bottom panal:DNA methyltransferase 1 shRNA knockdown (KD). Nuclei were stained blue with DAPI;DNA methyltransferase 1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
Citation(0)
Flow cytometric analysis of DNA methyltransferase 1 expression in HepG2 cells using anti-DNA methyltransferase 1 antibody (Cat#63144, 1:2,000). Green, isotype control; red, DNA methyltransferase 1.
Immunocytochemical staining of HepG2 cells with DNA methyltransferase 1 antibody (Cat#63144, 1:1,000). Nuclei were stained blue with DAPI; DNA methyltransferase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-DNA methyltransferase 1 antibody (Cat#63144). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-DNA methyltransferase 1 antibody (Cat#63144, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit(Cat#716).
Western blotting analysis using anti-DNA methyltransferase 1 antibody (Cat#63144). DNA methyltransferase 1 expression in wild type (WT) and DNA methyltransferase 1 (DNMT1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-DNA methyltransferase 1 antibody (Cat#63144, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of DNA methyltransferase 1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-DNA methyltransferase 1 antibody (Cat#63144, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of Hela cells using anti-DNA methyltransferase 1 antibody (Cat#63144, 1:1,000), Top panel: wild-type (WT); Bottom panal:DNA methyltransferase 1 shRNA knockdown (KD). Nuclei were stained blue with DAPI;DNA methyltransferase 1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
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