Flow cytometric analysis of EIF3B expression in HepG2 cells using anti-EIF3B antibody (Cat#63215, 1:2,000). Green, isotype control; red, EIF3B.
Western blotting analysis using anti-EIF3B antibody (Cat#63215). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-EIF3B antibody (Cat#63215, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). EIF3B, eukaryotic translation initiation factor 3 subunit B.
Western blotting analysis using anti-EIF3B antibody (Cat#63215). EIF3B expression in wild type (WT) and EIF3B shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-EIF3B antibody (Cat#63215, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of EIF3B knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-EIF3B antibody (Cat#63215, 1:2,000) and analyzed using BD flow cytometer.
