Flow cytometric analysis of Checkpoint kinase 2 expression in HAP-1 cells using anti-Checkpoint kinase 2 antibody (Cat#63224, 1:2,000). Green, isotype control; red, Checkpoint kinase 2.
Immunocytochemical staining of HAP1 cells with anti-Checkpoint kinase 2 antibody (Cat#63224, 1:1,000) . Nuclei were stained blue with DAPI;Checkpoint kinase 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-checkpoint kinase 2 antibody (Cat#63224). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-checkpoint kinase 2 antibody (Cat#63224, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-checkpoint kinase 2 antibody (Cat#63224). Checkpoint kinase 2 expression in wild type (WT) and checkpoint kinase 2 (CHEK2) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-checkpoint kinase 2 antibody (Cat#63224, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of Checkpoint kinase 2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Checkpoint kinase 2 antibody (Cat#63224, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of Hela cells using anti-Checkpoint kinase 2 antibody (Cat#63224, 1:1,000), Top panel: wild-type (WT); Bottom panal: Checkpoint kinase 2 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Checkpoint kinase 2 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
