Flow cytometric analysis of HDAC3 expression in HepG2 cells using HDAC3 antibody (Cat#63243,1:2,000). Green, isotype control; red, HDAC3.
Immunocytochemical staining of HepG2 cells with HDAC3 antibody (Cat#63243, 1:1,000). Nuclei were stained blue with DAPI; HDAC3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-HDAC3 antibody (Cat#63243). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HDAC3 antibody (Cat#63243, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-HDAC3 antibody (Cat#63243). HDAC3 expression in wild type (WT) and HDAC3 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-HDAC3 antibody (Cat#63243, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of HDAC3 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-HDAC3 antibody (Cat#63243, 1:2,000) and analyzed using CytoFLEX.
