Flow cytometric analysis of CTH expression in HepG2 cells using anti-CTH antibody (Cat#63268, 1:2,000). Green, isotype control; red, CTH.
Immunocytochemical staining of HepG2 cells with anti-CTH antibody(Cat#63268, 1:1,000) . Nuclei were stained blue with DAPI; CTH was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-CTH antibody (Cat#63268). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CTH antibody (Cat#63268, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-CTH antibody (Cat#63268). CTH expression in wild type (WT) and CTH shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-CTH antibody (Cat#63268, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
