Flow cytometric analysis of RELB expression in C2C12 cells using anti-RELB antibody (Cat#63326, 1:2,000). Green, isotype control; red, RELB.
Immunocytochemical staining of C2C12 cells with anti-RELB antibody (Cat#63326, 1:1,000) . Nuclei were stained blue with DAPI; RELB was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-RELB antibody (Cat#63326). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RELB antibody (Cat#63326, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-RELB antibody (Cat#63326). RELB expression in wild-type (WT) and RELB shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-RELB antibody (Cat#63326, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).