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KD-Validated Anti-RELB Rabbit Monoclonal Antibody #63326

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Flow cytometric analysis of RELB expression in C2C12 cells using anti-RELB antibody (Cat#63326, 1:2,000). Green, isotype control; red, RELB.
Immunocytochemical staining of C2C12 cells with anti-RELB antibody (Cat#63326, 1:1,000) . Nuclei were stained blue with DAPI; RELB was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-RELB antibody (Cat#63326). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RELB antibody (Cat#63326, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-RELB antibody (Cat#63326). RELB expression in wild-type (WT) and RELB shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-RELB antibody (Cat#63326, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名:
    RELB
  • 货号:
    63326
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 650.00,¥ 1,350.00,¥ 2,500.00
Information
Product Name KD-Validated Anti-RELB Rabbit Monoclonal Antibody
Aliases RELB; RELB Proto-Oncogene, NF-KB Subunit; REL-B; V-Rel Avian Reticuloendotheliosis Viral Oncogene Homolog B (Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 3); Transcription Factor RelB ; V-Rel Reticuloendotheliosis Viral Oncogene Homolog B, Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 3; I-REL; IMD53; I-Rel; IREL
Background

Gene Name: RELB

NCBI Gene Entry: 5971

UniProt Entry: Q01201

Application Information

Molecular Weight: Predicted, 62 kDa, observed, 70 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB5070

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human Rel B
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of RELB expression in C2C12 cells using anti-RELB antibody (Cat#63326, 1:2,000). Green, isotype control; red, RELB.
  • Immunocytochemical staining of C2C12 cells with anti-RELB antibody (Cat#63326, 1:1,000) . Nuclei were stained blue with DAPI; RELB was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-RELB antibody (Cat#63326). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RELB antibody (Cat#63326, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-RELB antibody (Cat#63326). RELB expression in wild-type (WT) and RELB shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-RELB antibody (Cat#63326, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
Flow cytometric analysis of RELB expression in C2C12 cells using anti-RELB antibody (Cat#63326, 1:2,000). Green, isotype control; red, RELB.
Immunocytochemical staining of C2C12 cells with anti-RELB antibody (Cat#63326, 1:1,000) . Nuclei were stained blue with DAPI; RELB was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-RELB antibody (Cat#63326). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RELB antibody (Cat#63326, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-RELB antibody (Cat#63326). RELB expression in wild-type (WT) and RELB shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-RELB antibody (Cat#63326, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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