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KD-Validated Anti-STAU1 Rabbit Monoclonal Antibody #63331

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Flow cytometric analysis of STAU1 expression in HT-1080 cells using anti-STAU1antibody (Cat#63331, 1:2,000). Green, isotype control; red, STAU1.
Immunocytochemical staining of HT-1080 cells with anti-STAU1 antibody(Cat#63331, 1:1,000) . Nuclei were stained blue with DAPI; STAU1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-STAU1 antibody (Cat#63331). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-STAU1 antibody (Cat#63331, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). STAU1, staufen double-stranded RNA binding protein 1.
Western blotting analysis using anti-STAU1 antibody (Cat#63331). STAU1 expression in wild type (WT) and STAU1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-STAU1 antibody (Cat#63331, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of STAU1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-STAU1 antibody (Cat#63331, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of Hela cells using anti-STAU1 antibody (Cat#63331, 1:1,000), Top panel: wild-type (WT); Bottom panal:STAU1 shRNA knockdown (KD). Nuclei were stained blue with DAPI;STAU1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
  • 基因名：
    STAU1
  • 货号：
    63331
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-STAU1 Rabbit Monoclonal Antibody
Aliases STAU1; Staufen Double-Stranded RNA Binding Protein 1; PPP1R150; STAU; Double-Stranded RNA-Binding Protein Staufen Homolog 1; Protein Phosphatase 1, Regulatory Subunit 150; Staufen, RNA Binding Protein, Homolog 1 (Drosophila); Staufen (Drosophila, RNA-Binding Protein); Staufen, RNA Binding Protein (Drosophila); Staufen, RNA Binding Protein, Homolog 1
Background

Gene Name: STAU1

NCBI Gene Entry: 6780

UniProt Entry: O95793
Application Information

Molecular Weight: Predicted, 63 kDa, observed, 63 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB5095

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human Staufen
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of STAU1 expression in HT-1080 cells using anti-STAU1antibody (Cat#63331, 1:2,000). Green, isotype control; red, STAU1.
  • Immunocytochemical staining of HT-1080 cells with anti-STAU1 antibody(Cat#63331, 1:1,000) . Nuclei were stained blue with DAPI; STAU1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-STAU1 antibody (Cat#63331). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-STAU1 antibody (Cat#63331, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). STAU1, staufen double-stranded RNA binding protein 1.
  • Western blotting analysis using anti-STAU1 antibody (Cat#63331). STAU1 expression in wild type (WT) and STAU1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-STAU1 antibody (Cat#63331, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Validation of STAU1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-STAU1 antibody (Cat#63331, 1:2,000) and analyzed using BD flow cytometer.
  • Immunocytochemical staining of Hela cells using anti-STAU1 antibody (Cat#63331, 1:1,000), Top panel: wild-type (WT); Bottom panal:STAU1 shRNA knockdown (KD). Nuclei were stained blue with DAPI;STAU1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
Citation(0)
  • 基因名：
    STAU1
  • 货号：
    63331
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of STAU1 expression in HT-1080 cells using anti-STAU1antibody (Cat#63331, 1:2,000). Green, isotype control; red, STAU1.
Immunocytochemical staining of HT-1080 cells with anti-STAU1 antibody(Cat#63331, 1:1,000) . Nuclei were stained blue with DAPI; STAU1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-STAU1 antibody (Cat#63331). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-STAU1 antibody (Cat#63331, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). STAU1, staufen double-stranded RNA binding protein 1.
Western blotting analysis using anti-STAU1 antibody (Cat#63331). STAU1 expression in wild type (WT) and STAU1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-STAU1 antibody (Cat#63331, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of STAU1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-STAU1 antibody (Cat#63331, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of Hela cells using anti-STAU1 antibody (Cat#63331, 1:1,000), Top panel: wild-type (WT); Bottom panal:STAU1 shRNA knockdown (KD). Nuclei were stained blue with DAPI;STAU1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
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